No. We automatically take care of this issue. We realize that it could be very likely that your subscribers might be interested in subscribing to more than one of your lists and because of this, when you send one campaign that targets multiple Subscriber Lists, we automatically prevent delivery duplication for that campaign.
If the recipient (who appears across more than one list you are sending to) unsubscribes, they will be removed from each list you sent to that they appear in.
If you were to send to each Subscriber List separately, we are unable to determine that there are duplicates. Subscribers in more than one of these Subscriber Lists would get multiple campaigns in their inbox.